Research Center for Resource Insects and Biotechnology

    Research Center was established in 1978, with former name entitled Research Group for Entomopathogenic Nematodes. The Center was renamed as the Biotechnology Research Group in 2000 and as Resource Insects and Biotechnology since 2009.

Research Areas

   (1) The research, industrialization and application of the novel biological insecticide - entomopathogenic nematodes.

   (2) The research and utilization of resource insects (medicinal insects-the Hepialidae insects, pollinators-honey bee, feed insects-house fly) and their functional genes.

   (3) The research and application of biotechnology (fermentation engineering, genetic engineering, biochemistry etc.). 

Research Progress

    In the post 20 years, the Research Center has undertaken more than 80 important research projects, including international cooperation projects [such as projects from Australian Centre for International Agricultural Research (ACIAR), International Foundation for Science (IFS), the European Union Fifth and Seventh Framework Program (FP5 and FP7), Flemish Interuniversity Council and UK Department for International Development (DFID)], projects supported by the National Natural Science Foundation of China, Natural Science Foundation of Guangdong Province, and other projects supported by local government.

    The research center has obtained 9 academic prizes from national and provincial levels; 83 applied national invention patents (including55 authorized patents and 6 international authorized patents; more than 160 research papers including more than 70 papers in SCI source; 4 English books with chapters in “Microbial Control of Insect and Mite Pest”, “Entomopathology Nematology”, “Application of Entomopathogenic Nematodes”, “Mass production of Beneficial organisms”, and 1 Chinese book with chapter in “Biological Control for Pest”; 18 corporate standards; and 38 trained Master students, 11 PhD students, and 3 postdoctoral fellows since 2001.

科研方向

    主要從事生物殺蟲劑產業化, 資源昆蟲研發, 以及天然活性代謝產物發現與藥效活性挖掘。

科研團隊組成

    中心主任：韓日疇博士、研究員（二級），中國昆蟲學會副理事長，廣東省昆蟲學會理事長；中國科學院大學、中山大學和比利時根特大學等博士生導師。從事生物農藥和資源昆蟲研發。

   二級PI:

    曹莉碩士、研究員，碩士研究生導師；從事生物農藥產業化和冬蟲夏草研發。

    李文峰博士、副研究員，碩士研究生導師；從事蜜蜂化學通訊和病蟲害研究。

    張祎博士、副研究員，碩士研究生導師；從事要從事蜜蜂病理與病蟲害防治研究。

科研成果概況、近五年科研成果列表

    近五年發表的主要學術論文：(*為通訊作者)

    1. Long H, Qiu X, Cao L, Liu G, Rao Z, Han R*. Toxicological safety evaluation of  the cultivated Chinese cordyceps. Journal of Ethnopharmacology, doi.org/10.1016/j.jep.2020.113600 (IF=3.69, 藥物學1區)

    2. Wu H, Rao Z, Cao L, De Clercq P*, Han R*. Infection of Ophiocordyceps sinensis fungus causes dramatic changes in the microbiota of its Thitarodes host, Frontiers in Microbiology , doi.org/10.3389/fmicb.2020.577268 (IF=4.235, 生物2區)

    3. Liu G, Lv Z, Wu Q, Zhou Z, Zhang G, Wan F*, Yan Y. The Bactrocera dorsalis caspase1 gene is expressed throughout development and required for female fertility, Pest Management Science, 2020, DOI: 10.1002/ps.5966. (IF=3.252, 生物1區)

    4. Liu G, Cao L, Rao Z, Qiu X, Han R*. Identification of the genes involved in growth characters of medicinal fungus Ophiocordyceps sinensis based on Agrobacterium tumefaciens-mediated transformation. Applied Microbiology and Biotechnology, 2020, 104(6): 2663-2674. doi: 10.1007/s00253-020-10417-1. (IF=3.67, 工程技術2區)

    5. Qiu X, Cao L, Han R*. Analysis of volatile components in different Ophiocordyceps sinensis and insect host products. Molecules, 2020, 25: pii: E1603. doi: 10.3390/molecules25071603. (IF=3.06, 化學3區)

    6. Liu G, Cao L, Qiu X, Han R*. Quorum sensing activity and hyphal growth by external stimuli in the entomopathogenic fungus Ophiocordyceps sinensis. Insects, 2020, 11(4). pii: E205. doi: 10.3390/insects11040205. (IF=2.137, 農林科學2區)

    7. Wang Tao, Keller A. Michael*. Larger is better in the parasitoid Eretmocerus warrae (Hymenoptera: Aphelinidae). Insects, 2020, 11:39. (IF=2.137, 農林科學2區)

    8. Rao Z, Cao L, Wu H, Qiu X, Liu G, Han R*. Comparative transcriptome analysis of Thitarodes armoricanus in response to the entomopathogenic fungi Paecilomyces hepiali and Ophiocordyceps sinensis. Insects, 2020, 11(1): pii: E4. doi: 10.3390/insects11010004. (IF=2.137, 農林科學2區)

    9. Yan X, Shahid Arain M, Lin Y, Gu X, Zhang L, Li J, Han R*. Efficacy of entomopathogenic nematodes against the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae). Journal of Economic Entomology, 2020, 113(1): 64-72. doi: 10.1093/jee/toz262. (IF=1.779, 昆蟲學2區)

    10. Li W, Wang C, Huang ZY, Chen Y, Han R*. Reproduction of distinct Varroa destructor genotypes on honey bee worker brood. Insects, 2019, 10(11): pii: E372. doi: 10.3390/insects10110372. (IF=2.137, 農林科學2區)

    11. Rao Z, Cao L, Qiu X, Han R*. Comparative transcriptome analysis reveals molecular strategies of ghost moth Thitarodes armoricanus in response to hypoxia and anoxia. Journal of Insect Physiology, 2019, 112, 23-34. doi: https:// doi.org/10.1016/j.jinsphys.2018.11.001. (IF=2.862, 農林科學2區)

    12. Zhang Y, Han R*. Insight into the salivary secretome of the Varroa destructor and salivary toxicity to Apis cerana. Journal of Economic Entomology, 2019, 112(2):505-514. doi: 10.1093/jee/toy224. (IF=1.779, 昆蟲學2區)

    13. Yan X, Zhao G, Han R*. Integrated management of chive gnats (Bradysia odoriphaga Yang & Zhang) in chives using entomopathogenic nematodes and low-toxicity insecticides. Insects, 2019, 10(6): pii: E161. doi: 10.3390/insects10060161. (IF=2.137, 農林科學2區)

    14. Liu G, Han R, Cao L*. Artificial cultivation of the Chinese cordyceps from injected ghost moth larvae. Environmental Entomology, 2019, 48(5): 1088-1094. (IF=1.45, 昆蟲學3區)

    15. Liu G, Qiu X, Cao L, Han R*. Scratching stimuli of mycelia influence fruiting body production and ROS-scavenging gene expression of Cordyceps militaris. Mycobiology, 2018, 46(4): 382–387. (IF=1.369, 真菌學4區) 

    16. Zhang Y, Han R*. A saliva protein of Varroa mites contributes to the toxicity toward Apis cerana and the DWV Elevation in A. mellifera. Scientific Reports, 2018, 8(1): 3387. doi: 10.1038/s41598-018-21736-9. (IF=4.259, 綜合性期刊3區)

    17. Yan X, Lin Y, Huang Z, Han R*. Characterization of biological and biocontrol traits of entomopathogenic nematodes promising for control of striped flea beetle (Phyllotreta striolata). Nematology, 2018, DOI: 10.1163/15685411-00003155. (IF=1.216, 動物學3區)

    18. Guo W, Yan X, Zhao G, Han R*. Increased efficacy of entomopathogenic nematode-insecticide combinations against Holotrichia oblita. Journal of Economic Entomology, 2017, 110(1): 41-51. (IF=1.779, 昆蟲學2區)

    19. Guo W, Yan X, Han R*. Adapted formulations for entomopathogenic nematodes, Steinernema and Heterorhabditis spp. Nematology, 2017, 19(5): 587-596. (IF=1.216,動物學3區)

    20. Liu G, Qiu X, Cao L, Zhang Y, Han R*. Evaluation of reference genes for reverse transcription quantitative PCR Studies of physiological responses in the ghost moth, Thitarodes armoricanus (Lepidoptera, Hepialidae). PLOS ONE, 2016, 11(7): e0159060. doi: 10.1371/journal.pone.0159060. (IF=2.806, 綜合性期刊3區)

    21. Zhang J, Zhang Y, Han R*. The high-throughput production of dsRNA against sacbrood virus for use in the honey bee Apis cerana (Hymenoptera: Apidae). Virus Genes. 2016, 52(5): 698-705. doi: 10.1007/s11262-016-1346-6. (IF=1.616, 病毒學4區)

    22. Zhan Z, Qiu X, Han R*. Horizontal transfer of the C-termini of tccC genes in Photorhabdus and Xenorhabdus. Genes & Genomics, 2016, 38(8): 685-692. DOI: 10.1007/s13258-016-0410-x. (SCI，分子生物學4區)

    23. Qiu X, Wu C, Cao L, Ehlers RU, Han R. Photorhabdus luminescens LN2 requires rpoS for nematicidal activity and nematode development. FEMS Microbiology Letters, 2016, 363(6): doi: 10.1093/femsle/fnw035. (IF=1.765, 微生物4區)

    24. Yan X, Waweru B, Qiu X, Hategekimana A, Kajuga J, Li H, Edgington S, Umulisa C, Han R*, Toepfer S. New entomopathogenic nematodes from semi natural and small-holder farming habitats of Rwanda. Biocontrol Science and Technology, 2016, 26 (6): 820-834. (SCI，昆蟲學4區)

    25. Tao Z, Cao L, Zhang Y, Ye Y, Han R*. Laboratory Rearing of Thitarodes armoricanus and Thitarodes jianchuanensis (Lepidoptera: Hepialidae), Hosts of the Chinese Medicinal Fungus Ophiocordyceps sinensis (Hypocreales: Ophiocordycipitaceae). Journal of Economic Entomology, 2016, 109(1): 176-781. (IF=1.824, 昆蟲學2區 )

    26. Cao L, Ye Y, Han R*. Fruiting body production of the medicinal Chinese caterpillar mushroom, Ophiocordyceps sinensis (Ascomycetes), in artificial medium. International Journal of Medicinal Mushrooms, 2015, 17(11): 1017–1022. (IF=1.357, 真菌學4區)

    27. Jiang K. Han R*. Rhf1 gene is involved in the fruiting body production of Cordyceps militaris fungus. Journal of Industrial Microbiology & Biotechnology, 2015, 42(8): 1183–1196. (IF=2.745, 生物工程與應用微生物3區)

    28. Zheng Z, Qiu X. Han R*. Identification of the genes involved in the fruiting body production and cordycepin formation of Cordyceps militaris Fungus. Mycobiology, 2015, 43(1): 37-42. (SCI, 真菌學4區)

    29. Guo W, Yan X, Zhao G, Chen J, Han R*. Efficacy of entomopathogenic Steinernema and Heterorhabditis nematodes against Holotrichia oblita. Journal of Pest Science, 2015, 88: 359-368. (IF=3.107, 昆蟲學1區)

專著編寫

     1.Peters A, Han R, Yan X, Leite LG. Chapter 10. Production of Entomopathogenic Nematodes. In: Lacey L.A. (ed.) Microbial Control of Insect and Mite Pest4s: From Theory to Practice. 2017. Elsevier.

    2.Shapiro-Ilan DI, Han R, Qiu X. Chapter 10 - Production of Entomopathogenic Nematodes. In: Mass Production of Beneficial Organisms, Academic Press, 2014, Pages 321-355.

    3.Shapiro-Ilan DI, Duncan LW, Lacey LA and Han R. Orchard crops. In: Nematodes As Biocontrol Agents (edited by Grewal PS, Ehlers R-U and Shapiro-Ilan DI), CABI Publishing, 2005, Pages 215-229. 2004.03

    4.Gaugler R, Han R. Production technology. In: Entomopathogenic Nematology (edited by Gaugler R), CABI Publishing, 2001, Pages 289-310. 2001.03

    5.韓日疇, 丘雪紅. 昆蟲病原線蟲的利用.《害蟲生物防治》林乃銓主編, 科學出版社，2010, p.287-314. 2010.04

發明專利

近五年授權發明專利 22（其中國際授權專利6件）：

    1.郭文秀，韓日疇，顏珣. 國家發明專利：腐殖土作為載體在貯存昆蟲病原線蟲中的應用. 專利號：ZL201710297018.6；授權公告日：2020.10.20.

    2.陳鏡華，丘雪紅，韓日疇. 國家發明專利： 一種白蟻誘餌、白蟻誘殺裝置及其制造方法. 專利號：ZL2017101216308；授權公告日：2020.10.15.

    3.劉桂清，曹莉，丘雪紅，韓日疇. 國家發明專利：一種冬蟲夏草菌液體培養基及高效獲得冬蟲夏草寄主昆蟲感染用芽生孢子的方法. 專利號：ZL201810513326.2；授權公告日：2020.10.12.

    4.張祎，韓日疇. 國家發明專利：一種蜜蜂西奈湖病毒基因組擴增引物和擴增方法及其應用. 專利號：ZL201710279245.5；授權公告日：2020.07.28.

    5.張祎，韓日疇. 國家發明專利：一種快速檢測蜜蜂絲狀病毒的檢測引物組、檢測試劑盒和檢測方法. 專利號：ZL201710278715.7；授權公告日：2020.07.07.

    6.Qiu X, Han R. 美國發明專利：Artificial diet for ghost moth larvae and preparation method therefor. 美國專利號：US10681921B2；授權日：2020.06.16. 

    7.Cao L, Han R. 美國發明專利：Artificial feeding method at low altitude for host insect ghost moth of Ophiocordyceps sinensis. 美國專利號：US10412941B2, 授權日：2019.09.17. 

    8.Cao L, Han R. 美國發明專利：Method for artificial cultivation of Ophiocordyceps sinensis fruiting bodies. 美國專利號：US10400209B2, 授權日：2019.09.03. 

    9.Qiu X, Han R.日本發明專利：Artificial diet for ghost moth larvae and preparation method therefor.日本特許證，特許第6535419號；授權日：2019.06.07.

    10.顏珣，韓日疇. 國家發明專利：一種快速獲得小卷蛾斯氏線蟲整齊齡期感染期幼蟲的方法. 專利號：ZL201610257254.0；授權公告日：2018.10.23.

    11.丘雪紅，韓日疇. 國家發明專利：一種蝙蝠蛾幼蟲的人工飼料及其制備方法. 專利號：ZL201610938312.6；授權公告日：2018.07.20.

    12.張祎，韓日疇. 國家發明專利：一種狄斯瓦螨唾液混合物及其制備方法和應用. 專利號：ZL201410217111.8；授權公告日：2018.02.23.

    13.張祎，韓日疇. 國家發明專利：一種狄斯瓦螨毒性蛋白及其編碼基因和應用. 專利號：ZL201410217113.7；授權公告日：2018.01.19.

    14.Cao L, Han R. 日本發明專利：Method for artificial cultivation of Ophiocordyceps sinensis fruiting bodies. PCTCN2014092747.日本特許證，特許第6143245號；授權日：2017.05.19.

    15.Cao L, Han R. 日本發明專利：Artificial feeding method at low altitude for host insect ghost moth of Ophiocordyceps sinensis. PCTCN2014092751. 日本受理號：51601181471，日本特許證，特許第6143246號；授權日：2017.05.19

    16.丘雪紅，韓日疇. 國家發明專利：珠芽蓼根在制備小菜蛾與斜紋夜蛾殺蟲劑中的應用. 專利號：ZL201510680995.5；授權公告日：2017.11.21.

    17.江克清，韓日疇. 國家發明專利：一種能提高蛹蟲草感染寄主昆蟲能力的蛹蟲草工程菌. 專利號：ZL201410218655.6；授權公告日：2017.01.25.

    18.丘雪紅，胡素麗，韓日疇. 國家發明專利：轉錄因子DksA基因的新用途. 專利號：ZL201410328197.1；授權公告日：2016.08.17.

    19.陳鏡華，韓日疇，顏珣.  國家發明專利：一種食用/藥用/飼用昆蟲的干燥設備. 專利號：ZL201410103884.3；授權公告日：2016.04.20.

    20.江克清，韓日疇. 國家發明專利：一種蛹蟲草Rhf1基因失活的工程菌以及蛹蟲草Rhf1基因的應用. 專利號：ZL201410217012.X；授權公告日：2016.02.24.

    21.曹莉，韓日疇. 國家發明專利：一種冬蟲夏草寄主昆蟲蝙蝠蛾的人工低海拔飼養方法. 專利號：ZL 201410413333.7；授權公告日：2015.08.19. 

    22.曹莉，韓日疇. 國家發明專利：一種冬蟲夏草子實體人工栽培方法. 專利號：ZL201410289703.0；授權公告日：2015.05.20.

科技獎項

    1.2016年度廣東發明人獎，韓日疇，授獎時間：2017.07. 

    2.廣東省科學技術獎一等獎：地下和蛀干害蟲病原線蟲的系統研發和應用；授獎時間：2014.04, 第一完成單位.

    3.國家科技技術進步獎二等獎：有機固體廢棄物資源化與能源化綜合利用系列技術及應用；授獎時間：2011.12.23, 第五完成單位.

    4.國家科技技術進步獎二等獎：桔小實蠅持續控制基礎研究及關鍵技術集成創新與推廣；授獎時間：2009.12.23, 第七完成單位.

    5.山東省科學技術獎科技進步獎三等獎：昆蟲病原線蟲防治韭蛆等園藝害蟲研究與開發；授獎時間：2007.04.29. 第二完成單位.

    6.廣東專利獎優秀獎：韓日疇 李麗英, 一種昆蟲病原線蟲的液體培養方法.

    7.廣東省科學技術獎二等獎：昆蟲病原線蟲的液體發酵生產; 授獎時間：2005.05, 第一完成單位.

團隊照片

科研成果或獎項照片（一張）